3 edition of Genetic modification of hematopoietic stem cells found in the catalog.
Genetic modification of hematopoietic stem cells
Includes bibliographic references and index.
|Statement||edited by Christopher Baum|
|Series||Methods in molecular biology -- 506, Springer protocols|
|LC Classifications||QP92 .G458 2009|
|The Physical Object|
|Pagination||xxi, 494 p.  p. of plates :|
|Number of Pages||494|
|ISBN 10||9781588299802, 9781597454094|
|LC Control Number||2008939908|
The book is a fascinating, and highly readable, and takes a unique look at the dramatic advances in technology that allow us to edit the human genome in ways that could allow us to do more than just create “designer babies”, it could ultimately help us . Free 2-day shipping. Buy Methods in Molecular Biology: Genetic Modification of Hematopoietic Stem Cells: Methods and Protocols (Hardcover) at
Stem cell, an undifferentiated cell that can divide to produce some offspring cells that continue as stem cells and some cells that are destined to differentiate (become specialized). Stem cells are an ongoing source of the differentiated cells that make up the tissues and organs of animals and plants. There is great interest in stem cells because they have potential in the development of. Gene therapy (also called human gene transfer) is a medical field which focuses on the utilization of the therapeutic delivery of nucleic acid into a patient's cells as a drug to treat disease. The first attempt at modifying human DNA was performed in by Martin Cline, but the first successful nuclear gene transfer in humans, approved by the National Institutes of Health, was performed .
CRISPR-Cas technology is revolutionizing cell biology due to the ease and efficiency by which it enables the genetic manipulation of mammalian cells. Through targeted modification of specific genes or regulatory regions, researchers can now rapidly generate precise genetic models to study normal and diseased cell physiology. Methods of Genetic Manipulation. Genetic Modification Approaches. Summary. Addendum. Hematopoietic Stem Cells. Embryonic Stem Cells and Embryonic Hematopoiesis. Blood Formation in Embryoid Bodies. "The Handbook of Stem Cells, edited by Robert Lanza and colleagues, is an ambitious new text that achieves extraordinary completeness and.
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In Genetic Modification of Hematopoietic Stem Cells: Methods and Protocols, leading scientists in the field provide a compendium of protocols which cover the subject comprehensively, from the purification and culture of various types of hematopoietic cells for subsequent genetic modification by vector development and technical issues of small.
All hematopoietic cell types are of major interest in this context, since the modification of the hematopoietic stem cell population may potentially give rise to a completely transgenic hematopoiesis with the potential to cure genetic disorders or fight severe chronic infections, and the targeting of mature cells such as lymphocytes or antigen Author: Christopher Baum.
Free Online Library: Genetic modification of hematopoietic stem cells; methods and protocols.(Brief article, Book review) by "SciTech Book News"; Publishing industry Library and information science Science and technology, general Books Book reviews.
In particular, this impact includes: a) differentiation of human embryonic stem cells into various cell types, such as neurons, cardiac, vascular, hematopoietic, pancreatic, hepatic, and placental cells, b) the derivation of new cell lines under alternative conditions, c) and the establishment of protocols that allow the genetic modification of.
All hematopoietic cell types are of major interest in this context, since the modification of the hematopoietic stem cell population may potentially give rise to a completely transgenic hematopoiesis with the potential to cure genetic disorders or fight severe chronic infections, and the targeting of mature cells such as lymphocytes or antigen Price: $ hematopoietic stem and progenitor cells for research use only.
not intended for human or animal diagnostic or therapeutic uses. 3 stemcell technologies inc.s ualit management sstem is certified to iso medical device standards.
Immunomagnetic enrichment of human and mouse hematopoietic stem cells for gene therapy applications / Guillermo Guenechea, Jose C. Segovia, and Juan A. Bueren --Isolation of human and mouse hematopoietic stem cells / Yuk Yin Ng [and others] --Murine hematopoietic stem cell transduction using retroviral vectors / Ute Modlich [and others.
Unique and cutting-edge, Genetic Modification of Hematopoietic Stem Cells: Methods and Protocols is an ideal, thorough resource to promote further research and the implementation of investigator-driven clinical studies using gene-modified hematopoietic cells.\/span>\"@ en\/a> ; \u00A0\u00A0\u00A0\n schema:description\/a> \" In situ (in vivo.
Kawahara, Y. Shiozawa, in Reference Module in Biomedical Sciences, Hematopoietic Stem Cell Markers. Since hematopoietic stem cells are an extremely rare population in the marrow (approximately 1/10 bone marrow cells), the identification of hematopoietic stem cells in the marrow is still challenging.
Historically, the functional assays, including in vitro colony. Genetic Modification of Murine Hematopoietic Stem Cells by Retroviruses. et al. () Retroviral-mediated transfer of the green fluores cent protein gene into murine hematopoietic cells facilitates scoring and selection of transduced progenitors in vitro and identification of genetically modified cells in vivo.
Genetic Modification of Cited by: 3. Genetic modification of stem cells can be carried out ex vivo, by transducing bone marrow or peripheral blood stem cell-rich fractions with viral vectors carrying therapeutic : Fulvio Mavilio.
With the incredible potential of gene transfer into hematopoietic stem cells, active research in this field has become critically important. In Genetic Modification of Hematopoietic Stem Cells: Methods and Protocols, leading scientists in the field provide a compendium of protocols which cover the subject comprehensively, from the purification and culture of various types of.
Adult hematopoietic stem cells (HSCs) are a rare population of cells that are present in the bone marrow (BM) and are the responsible for the generation of all mature blood cells, including erythrocytes, platelets, and immune cells.
This population represents less than % of the total BM and is the only one capable of self-renewal, being Author: Sergio López-Manzaneda, Sara Fañanas-Baquero, Virginia Nieto Romero, Francisco-Jose Roman-Rodríguez.
The many approaches described employ not only molecular and cell biology techniques, but also animal model systems, and include hematopoietic stem cell expansion protocols and methods for the purification and genetic modification of stem cells.
The combination of genetic modification and hematopoietic stem cell (HSC) transplantation may provide the necessary means to develop an alternative treatment option to conventional antiretroviral therapy. As HSCs give rise to all hematopoietic cell types susceptible to HIV infection, modification of HSCs is an ideal strategy for the development of infection-resistant Cited by: 6.
Genetic marking of hematopoietic stem and endothelial cells: identification of the Tmtsp gene encoding a novel cell surface protein with the thrombospondin-1 domain.
Takayanagi S(1), Hiroyama T, Yamazaki S, Nakajima T, Morita Y, Usui J, Eto K, Motohashi T, Shiomi K, Keino-Masu K, Masu M, Oike Y, Mori S, Yoshida N, Iwama A, Nakauchi by: Genetic modification, or gene transfer, represents a method of treatment for several diseases. It has been used extensively in the context of cardiovascular diseases; however, its role in the context of metabolic diseases, such as diabetes and obesity, has remained largely unexplored.
In this chapter, we will review the use of adult stem cells, focusing on endothelial progenitor cells Cited by: 3. Murine Hematopoietic Stem Cell Transduction Using Retroviral Vectors 23 Ute Modlich, Axel Schambach, ZhixiongLi, and Bernhard Schiedlmeier 4.
Genetic Modification of Human Hematopoietic Cells: Preclinical Optimization of Oncoretroviral-mediated. A hematopoietic stem cell is a cell isolated from the blood or bone marrow that can renew itself, can differentiate to a variety of specialized cells, can mobilize out of the bone marrow into circulating blood, and can undergo programmed cell death, called apoptosis—a process by which cells that are detrimental or unneeded self-destruct.
Gene correction of hematopoietic stem cells by addition strategies and their drawbacks Gene therapy can be achieved by the delivery of genetic material into cells affected by a disease.
Hematopoietic stem cells have an enormous clinical importance for bone marrow transplantation, hematopoietic gene therapy, solid organ transplantation, and somatic tissue regeneration.
In Hematopoietic Stem Cell Protocols, a group of leading stem-cell investigators describe in detail the key laboratory methods for investigating this critically important cell type.4/5(1). One paradigmatic example is the genetic modification of hematopoietic stem and progenitor cells (HSPCs).
These are rare nucleated cells which reside in a specialized microenvironment within the bone marrow, and have the potential to self-renew and/or differentiate into all hematopoietic by: 5.Under steady-state conditions (normal hematopoietic turnover and an intact bone marrow niche), the majority of HSCs cycles slowly, yet continuously For genetic modification, HSCs are either harvested from peripheral blood or bone marrowThe yield and biologic features of cells from these sources differ depending on the use of mechanical Cited by: